4UVB

LSD1(KDM1A)-CoREST in complex with 1-Methyl-Tranylcypromine (1S,2R)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	I 2 2 2
Unit cell lengths	119.160, 180.040, 234.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.22188
Rwork	0.222
R-free	0.23558
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v1d
RMSD bond length	0.005
RMSD bond angle	0.888
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0027)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.090	0.540
Number of reflections	62197
<I/σ(I)>	9.3	2.5
Completeness [%]	99.9	99.8
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		pH 6.5