4UUV
Structure of the DNA binding ETS domain of human ETV4 in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 10 |
| Detector technology | PIXEL |
| Collection date | 2014-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.651, 46.133, 171.150 |
| Unit cell angles | 90.00, 96.69, 90.00 |
Refinement procedure
| Resolution | 40.970 - 2.800 |
| R-factor | 0.2052 |
| Rwork | 0.203 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uno |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.521 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.260 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.050 | 0.460 |
| Number of reflections | 32705 | |
| <I/σ(I)> | 11.6 | 1.8 |
| Completeness [%] | 94.9 | 92.2 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG3350, 0.2M MG CL, 0.1M BIS TRIS |
