4UUQ
Crystal structure of human mono-glyceride lipase in complex with SAR127303
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-20 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 86.250, 126.260, 138.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.320 - 2.360 |
| R-factor | 0.215 |
| Rwork | 0.210 |
| R-free | 0.25910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jw8 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT (2.5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.320 | 2.500 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.010 | 0.450 |
| Number of reflections | 20609 | |
| <I/σ(I)> | 17.7 | 2.6 |
| Completeness [%] | 95.2 | 97.1 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 277 | MES BUFFER 50MM PH6.0, MPD 40%(V/V) AT 4C |
