4UU9
Crystal structure of the human c5a in complex with MEDI7814 a neutralising antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.854, 117.472, 126.457 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.070 - 2.120 |
| R-factor | 0.17595 |
| Rwork | 0.175 |
| R-free | 0.20163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.174 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 126.000 | 2.240 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.130 | 0.590 |
| Number of reflections | 48925 | |
| <I/σ(I)> | 19 | 8.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.2 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG10K, 8% ETHYLENE GLYCOL, 100MM HEPES PH 7.5 |
