4UT6
Crystal structure of dengue 2 virus envelope glycoprotein in complex with the Fab fragment of the broadly neutralizing human antibody EDE2 B7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 101.277, 58.980, 191.573 |
| Unit cell angles | 90.00, 96.20, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1OKE 3KDM AND 3H0T |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.340 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.110 | 0.640 |
| Number of reflections | 37500 | |
| <I/σ(I)> | 9.2 | 1.6 |
| Completeness [%] | 99.3 | 99 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100MM TRIS PH 8.5, 16% (W/V) PEG 400, 200MM MGCL2, CRYOPROTECTED IN 22%(V/V) GLYCEROL |
