4USJ
N-acetylglutamate kinase from Arabidopsis thaliana in complex with PII from Chlamydomonas reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 171.430, 171.430, 171.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.360 - 2.850 |
| R-factor | 0.1903 |
| Rwork | 0.189 |
| R-free | 0.22002 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rd5 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.038 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.300 | 2.960 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.110 | 0.680 |
| Number of reflections | 39062 | |
| <I/σ(I)> | 12.6 | 2.33 |
| Completeness [%] | 99.2 | 99 |
| Redundancy | 5.1 | 5.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM NACL, 100 MM NA/K PHOSPHATE, PH 6.2, 50% (V/V) PEG 200 |
