4URW
The crystal structure of H-Ras and SOS in complex with ligands
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-06 |
| Detector | ADSC CCD |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 149.411, 149.411, 200.344 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.390 - 2.760 |
| R-factor | 0.1842 |
| Rwork | 0.182 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bkd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.010 |
| Data reduction software | MOSFLM |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 119.770 | 2.840 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmerge | 0.100 | 0.500 |
| Number of reflections | 29350 | |
| <I/σ(I)> | 12.7 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.2M SODIUM FORMATE, 2% DMSO |
