4URK
PI3Kg in complex with AZD6482
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Collection date | 2005-02-11 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.179, 67.521, 106.167 |
| Unit cell angles | 90.00, 95.71, 90.00 |
Refinement procedure
| Resolution | 40.800 - 2.900 |
| R-factor | 0.2535 |
| Rwork | 0.250 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WWPDB ENTRY 1E8Y |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.800 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.060 | 0.380 |
| Number of reflections | 21692 | |
| <I/σ(I)> | 8.6 | 2 |
| Completeness [%] | 96.0 | 96 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% PEG3350, 0.1M HEPES (PH 7.5), 5% AMSO4, 10MM DTT |
