4UNA
THE CRYSTAL STRUCTURE OF I-DMOI IN COMPLEX WITH ITS TARGET DNA AT 2 DAYS INCUBATION IN 5MM MN (STATE 4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.517, 70.153, 106.704 |
| Unit cell angles | 90.00, 119.83, 90.00 |
Refinement procedure
| Resolution | 46.285 - 2.300 |
| R-factor | 0.1908 |
| Rwork | 0.190 |
| R-free | 0.21910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vs7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.194 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.280 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 0.480 |
| Number of reflections | 261170 | |
| <I/σ(I)> | 16 | 2 |
| Completeness [%] | 96.7 | 79.9 |
| Redundancy | 4.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | PH 6 |
