4UJ5
Crystal structure of human Rab11-Rabin8-FIP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.683, 108.202, 75.736 |
| Unit cell angles | 90.00, 102.83, 90.00 |
Refinement procedure
| Resolution | 43.642 - 2.604 |
| R-factor | 0.2174 |
| Rwork | 0.215 |
| R-free | 0.27160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yzk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.072 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmerge | 0.040 | 0.740 |
| Number of reflections | 24951 | |
| <I/σ(I)> | 14 | 1 |
| Completeness [%] | 94.0 | 84 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1M HEPES PH 7.0, 0.2M LITHIUM SULFATE AND 24% PEG 3350 |
