4UJ4
Crystal structure of human Rab11-Rabin8-FIP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.120, 161.701, 192.762 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.469 - 4.200 |
| R-factor | 0.2529 |
| Rwork | 0.252 |
| R-free | 0.26550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uj3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.151 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.400 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.130 | 0.670 |
| Number of reflections | 15003 | |
| <I/σ(I)> | 11 | 1.6 |
| Completeness [%] | 94.0 | 61 |
| Redundancy | 4.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 50MM MES PH 5.8, 0.2M AMMONIUM SULFATE AND 14% PEG 8000 |
