4UFS
Low resolution structure R-spondin-2 (Fu1Fu2) in complex with the ectodomains of LGR5 and ZNRF3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 188.609, 188.609, 165.292 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 133.370 - 4.800 |
| R-factor | 0.27215 |
| Rwork | 0.270 |
| R-free | 0.31262 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.848 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 5.370 |
| High resolution limit [Å] | 4.800 | 4.800 |
| Rmerge | 0.160 | 1.500 |
| Number of reflections | 7509 | |
| <I/σ(I)> | 8.5 | 1.5 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 5.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.100 M AMMONIUM ACETATE, 0.600 M SODIUM CHLORIDE, 0.050 M MES PH 6.0, 0.005 M MAGNESIUM SULPHATE |
