4UFR
Structure of the ectodomain of LGR5 in complex with R-spondin-2 (Fu1Fu2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-25 |
Detector | DECTRIS PILATUS |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 205.148, 59.777, 112.210 |
Unit cell angles | 90.00, 99.51, 90.00 |
Refinement procedure
Resolution | 110.670 - 2.200 |
R-factor | 0.20909 |
Rwork | 0.208 |
R-free | 0.26172 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.013 |
RMSD bond angle | 1.635 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 69.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.120 |
Number of reflections | 62167 |
<I/σ(I)> | 10.8 |
Completeness [%] | 90.6 |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 25 %W/V PEG3350, 0.200 M LITHIUM SULPHATE, 0.100 M BIS-TRIS PH 5.5 |