4UE0
Structure of the bovine atadenovirus type 4 fibre head protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.330, 48.190, 52.070 |
| Unit cell angles | 117.11, 95.56, 110.17 |
Refinement procedure
| Resolution | 44.150 - 1.170 |
| R-factor | 0.11699 |
| Rwork | 0.116 |
| R-free | 0.14595 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.549 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.150 | 1.210 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmerge | 0.030 | 0.350 |
| Number of reflections | 103174 | |
| <I/σ(I)> | 15.7 | 2.6 |
| Completeness [%] | 91.2 | 86.7 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% (W/V) PEG3350, 200 MM KSCN, 50 MM NACL, 10 MM TRIS-HCL PH 7.5, 5% (V/V) GLYCEROL | |||
| 1 | 20% (W/V) PEG3350, 200 MM KSCN, 50 MM NACL, 10 MM TRIS-HCL PH 7.5, 5% (V/V) GLYCEROL |
