4UCB
N-terminal globular domain of the RSV Nucleoprotein in complex with C- terminal peptide of the Phosphoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-12 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.520, 71.680, 178.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.790 |
R-factor | 0.1979 |
Rwork | 0.195 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wj8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.930 |
High resolution limit [Å] | 2.780 | 2.780 |
Rmerge | 0.120 | 0.420 |
Number of reflections | 45949 | |
<I/σ(I)> | 8.4 | 1.7 |
Completeness [%] | 94.7 | 71.1 |
Redundancy | 4.1 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |