4U8U
The Crystallographic structure of the giant hemoglobin from Glossoscolex paulistus at 3.2 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2009-07-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 272.680, 319.900, 333.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.655 - 3.200 |
| R-factor | 0.2165 |
| Rwork | 0.215 |
| R-free | 0.23520 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.962 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.112 | 0.500 |
| Number of reflections | 237062 | |
| <I/σ(I)> | 11.2 | 3.1 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 4.7 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG8000 10%, CaCl2 2.5 mM, TRIS 50 mM |
