4U8H
Crystal Structure of Mammalian Period-Cryptochrome Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 41 |
Unit cell lengths | 97.675, 97.675, 163.213 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.738 - 2.798 |
R-factor | 0.2088 |
Rwork | 0.205 |
R-free | 0.27780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mlp |
RMSD bond length | 0.009 |
RMSD bond angle | 1.345 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.738 |
High resolution limit [Å] | 2.798 |
Number of reflections | 37522 |
<I/σ(I)> | 18.8 |
Completeness [%] | 99.6 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M HEPES, 0.2 M NaCl, 15% PEG3350 |