4U7A
The carboxy-terminal domain of Erb1 is a seven-bladed beta-propeller that binds RNA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.024, 62.431, 158.218 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.073 - 1.600 |
| R-factor | 0.1605 |
| Rwork | 0.160 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h13 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.070 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.048 | |
| Number of reflections | 67582 | |
| <I/σ(I)> | 19.48 | 2.16 |
| Completeness [%] | 98.1 | 96.78 |
| Redundancy | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 10% PEG 8000, 8% ethylene glycol, 0.1M Hepes pH 7.5, 3% ethanol |
