4U73

HsMetAP(F309M) IN COMPLEX WITH (amino(phenyl)methyl)phosphonic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-03-26
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	47.477, 77.296, 48.271
Unit cell angles	90.00, 91.19, 90.00

Refinement procedure

Resolution	15.750 - 1.800
R-factor	0.172
Rwork	0.171
R-free	0.20100
RMSD bond length	0.021
RMSD bond angle	1.987
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	16.000
High resolution limit [Å]	1.800
Number of reflections	32452
<I/σ(I)>	3.58
Completeness [%]	99.5
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	15% PEGMME, 100mM HEPES PH 6