4U70
HsMetAP (F309M) in complex with (1-amino-2-cyclohexylethyl)phosphonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-02-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.636, 77.389, 48.575 |
| Unit cell angles | 90.00, 90.60, 90.00 |
Refinement procedure
| Resolution | 17.090 - 1.600 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.20700 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.283 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 18.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 46220 |
| <I/σ(I)> | 2.57 |
| Completeness [%] | 99.2 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 15% PEGMME, 100mM HEPES PH 6. |
