4U6C
HsMetAP in complex with [(1R)-1-amino-3-cyclopentylpropyl]phosphonic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-03-20 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.076, 77.299, 47.420 |
Unit cell angles | 90.00, 91.38, 90.00 |
Refinement procedure
Resolution | 21.000 - 1.910 |
R-factor | 0.186 |
Rwork | 0.183 |
R-free | 0.22900 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.907 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 22.640 |
High resolution limit [Å] | 1.870 |
Number of reflections | 26672 |
<I/σ(I)> | 2.94 |
Completeness [%] | 95.5 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 2000, potassium chloride, hepes, sodium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |