4U67
Crystal structure of the large ribosomal subunit (50S) of Deinococcus radiodurans containing a three residue insertion in L22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 85 |
Detector technology | CCD |
Collection date | 2012-07-10 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.873 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 169.720, 412.591, 696.974 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.991 - 3.650 |
R-factor | 0.228 |
Rwork | 0.226 |
R-free | 0.26990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zjr |
RMSD bond length | 0.007 |
RMSD bond angle | 1.192 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.710 |
High resolution limit [Å] | 3.650 | 3.650 |
Number of reflections | 261274 | |
<I/σ(I)> | 7.3 | 1.43 |
Completeness [%] | 95.9 | 92.1 |
Redundancy | 5.1 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | Magnesium chloride, HEPES, ammonium chloride, ethanol, 2-ethyl-1,3-hexandiol |