4U2N
Crystal structure of a complex of the Miz1- and Nac1 POZ domains.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 109.090, 115.190, 58.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.930 - 2.300 |
| R-factor | 0.2402 |
| Rwork | 0.238 |
| R-free | 0.28630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u2m |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.623 |
| Data reduction software | XDS (2.5.6) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.930 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.030 | 0.663 |
| Number of reflections | 33315 | |
| <I/σ(I)> | 24 | 2.4 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 0.2 % PEG 4000, 0.1 M sodium citrate pH 5.6 |
