4U1O

GluA2flip sLBD complexed with kainate and (R,R)-2b crystal form C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	57.310, 89.680, 48.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.770 - 1.850
R-factor	0.169
Rwork	0.167
R-free	0.20900
RMSD bond length	0.007
RMSD bond angle	1.206
Data reduction software	XDS
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.770
High resolution limit [Å]	1.850
Number of reflections	21890
<I/σ(I)>	17.4
Completeness [%]	99.1
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Na cacodylate, pH 6.5, 1.26 M ammonium sulfate