4U17

Swapped dimer of the human Fyn-SH2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9801
Spacegroup name	C 1 2 1
Unit cell lengths	88.965, 57.873, 101.188
Unit cell angles	90.00, 90.60, 90.00

Refinement procedure

Resolution	34.916 - 1.990
R-factor	0.2093
Rwork	0.207
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g83
RMSD bond length	0.012
RMSD bond angle	1.413
Data reduction software	iMOSFLM
Data scaling software	SCALA
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.920	2.100
High resolution limit [Å]	1.990	1.990
Rmerge		0.303
Number of reflections	35049
<I/σ(I)>	7.7	3.2
Completeness [%]	98.7	98.9
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	50 mM sodium phosphate pH 6.5