4U0S
Structure of Eukaryotic fic domain containing protein with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-09 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.040, 76.010, 92.030 |
| Unit cell angles | 90.00, 107.56, 90.00 |
Refinement procedure
| Resolution | 31.860 - 2.490 |
| R-factor | 0.2093 |
| Rwork | 0.207 |
| R-free | 0.24460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cuc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.860 |
| High resolution limit [Å] | 2.540 |
| Number of reflections | 32508 |
| <I/σ(I)> | 6.5 |
| Completeness [%] | 98.7 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 20% peg 3350, 200mM Na K tartrate, 100mM Bis-Tris-Propane 7.5 |
