4U0N
Structure of the Vibrio cholerae di-nucleotide cyclase (DncV) deletion mutant D-loop
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.968 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.605, 49.253, 122.233 |
Unit cell angles | 90.00, 98.66, 90.00 |
Refinement procedure
Resolution | 37.730 - 2.102 |
R-factor | 0.2198 |
Rwork | 0.217 |
R-free | 0.26420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u0l |
RMSD bond length | 0.006 |
RMSD bond angle | 0.881 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 120.000 |
High resolution limit [Å] | 2.100 |
Number of reflections | 50515 |
<I/σ(I)> | 21.1 |
Completeness [%] | 99.3 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 16% w/v PET 3350, 0.2M MgCl2, 100mM Tris-HCl |