4U0B
Hexamer HIV-1 CA in complex with CPSF6 peptide, P212121 crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96860 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 135.130, 135.890, 208.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.600 - 2.800 |
R-factor | 0.2311 |
Rwork | 0.230 |
R-free | 0.26190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u0a |
RMSD bond length | 0.004 |
RMSD bond angle | 0.739 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.600 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.069 | 0.588 |
Number of reflections | 89373 | |
<I/σ(I)> | 11.7 | |
Completeness [%] | 95.1 | 97.6 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | 20% v/v PEG 300, 10% v/v glycerol, 5% w/v PEG 8K, 0.1 M TRIS |