4TXZ

Crystal structure of Vibrio cholerae DncV cyclic AMP-GMP synthase in complex with nonhydrolyzable GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2014-05-12
Detector	PSI PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	70.041, 59.373, 103.498
Unit cell angles	90.00, 96.04, 90.00

Refinement procedure

Resolution	45.185 - 2.800
R-factor	0.2287
Rwork	0.228
R-free	0.24220
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.802
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.190
High resolution limit [Å]	2.800
Number of reflections	20746
<I/σ(I)>	5.8
Completeness [%]	97.9
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	30 mM HEPES-KOH pH 7.5, 200-300 mM Mg(OAc)2 and 20-22% PEG-3350