4TXY
Crystal structure of Vibrio cholerae DncV cyclic AMP-GMP synthase, a prokaryotic cGAS homolog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-05-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.167, 59.116, 102.539 |
| Unit cell angles | 90.00, 95.48, 90.00 |
Refinement procedure
| Resolution | 45.120 - 3.000 |
| R-factor | 0.2259 |
| Rwork | 0.225 |
| R-free | 0.25040 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.805 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.120 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 16701 | |
| <I/σ(I)> | 6.4 | 1.3 |
| Completeness [%] | 98.0 | 98.6 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 30 mM HEPES-KOH pH 7.5, 200-300 mM Mg(OAc)2 and 20-22% PEG-3350 |
