4TXR
Crystal structure of LIP5 N-terminal domain complexed with CHMP1B MIM and CHMP5 MIM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 193.15 |
Detector technology | CCD |
Collection date | 2013-04-23 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.99983 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.197, 60.567, 52.048 |
Unit cell angles | 90.00, 90.39, 90.00 |
Refinement procedure
Resolution | 28.668 - 1.000 |
R-factor | 0.1642 |
Rwork | 0.164 |
R-free | 0.17980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4txq |
RMSD bond length | 0.008 |
RMSD bond angle | 1.147 |
Refinement software | PHENIX ((phenix.refine: dev_1593)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.680 | 1.030 |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.050 | 0.270 |
Number of reflections | 99846 | |
<I/σ(I)> | 50.6 | 3.5 |
Completeness [%] | 87.4 | 46.1 |
Redundancy | 4.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277.15 | 19% PEG 4000, 0.025 M sodium acetate |