4TX9
Crystal structure of HisAp from Streptomyces sviceus with degraded ProFAR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 72.546, 72.546, 143.286 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.840 - 1.600 |
| R-factor | 0.1529 |
| Rwork | 0.152 |
| R-free | 0.18050 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.621 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_1613)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.078 | 1.000 |
| Number of reflections | 51524 | |
| <I/σ(I)> | 27.8 | 1.97 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 7.8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.2 M NaCl, 0.1 M imidazole/HCl, 0.4 M Na2HPO4/1.6 M K2HPO4, 10mg/ml protein, 2 mM ProFAR |
