4TWN
Human EphA3 Kinase domain in complex with Birb796
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00004 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.481, 38.421, 74.141 |
| Unit cell angles | 90.00, 101.50, 90.00 |
Refinement procedure
| Resolution | 48.403 - 1.706 |
| R-factor | 0.1786 |
| Rwork | 0.177 |
| R-free | 0.21270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dzq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.247 |
| Data reduction software | XDS (VERSION January 10, 2014 BUILT=20140115) |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 1.800 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.631 | |
| Number of reflections | 33440 | |
| <I/σ(I)> | 17.6 | 2.1 |
| Completeness [%] | 99.1 | 95.4 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M sodium cacodylate pH 6.5, 0.15M ammonium sulfate, 22.5% PEG 3350 |
