4TVX

Crystal structure of the E. coli CRISPR RNA-guided surveillance complex, Cascade

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-04
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	200.851, 214.541, 217.383
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.614 - 3.240
R-factor	0.2342
Rwork	0.234
R-free	0.26750
RMSD bond length	0.007
RMSD bond angle	1.231
Refinement software	PHENIX ((phenix.refine: dev_1760))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.614	3.356
High resolution limit [Å]	3.240	3.240
Rmerge	0.149	0.990
Number of reflections	148870
<I/σ(I)>	8.5	2.38
Completeness [%]	98.9	99.9
Redundancy	6.8	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	PEG 8000, sodium chloride, sodium thiocyanate, HEPES