4TV9
Tubulin-PM060184 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.200, 157.290, 180.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.044 - 2.000 |
| R-factor | 0.1815 |
| Rwork | 0.180 |
| R-free | 0.20770 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4i4t |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.709 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.050 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.104 | |
| Number of reflections | 199377 | |
| <I/σ(I)> | 23.7 | 1.1 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 37.6 | 20.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 6% PEG, 16% glycerol, 30 mM magnesium chloride, 30 mM calcium chloride, 0.1M MES/0.1M imidazole |
