Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 71.100, 71.100, 448.995 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.680 - 2.900 |
| R-factor | 0.2396 |
| Rwork | 0.235 |
| R-free | 0.33660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2euf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.316 |
| Data scaling software | XDS |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.680 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.410 | |
| Number of reflections | 16125 | |
| <I/σ(I)> | 28 | 6.2 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 11.8 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10 mM TRIS pH 7.9, 100 mM calcium acetate, 10 mM DTT, 8-12% PEG 3350, 100 mM NDSB-201 |
