4TT7

Crystal structure of human ALK with a covalent modification

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 21 21 21
Unit cell lengths	51.535, 57.157, 104.216
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.200 - 2.100
R-factor	0.19458
Rwork	0.192
R-free	0.24403
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xp2
RMSD bond length	0.010
RMSD bond angle	1.197
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.200	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.135	0.621
Number of reflections	18384
<I/σ(I)>	10.1	2.8
Completeness [%]	98.8	97.5
Redundancy	5.6	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	2 uL of 17 mg/mL protein (50 mM Bicine pH 8.4, 150 mM NaCl, 5 mM DTT) with 2 uL of 0.1 M TRIS hydrochloride, 0.2 M Sodium acetate trihydrate, 30% w/v Polyethylene glycol 4,000