4TSY
Crystal structure of FraC with lipids
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 151.337, 199.879, 120.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.650 - 3.140 |
| R-factor | 0.19967 |
| Rwork | 0.199 |
| R-free | 0.22123 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vwi |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.698 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.650 | 3.320 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Rmerge | 0.150 | 0.410 |
| Number of reflections | 30154 | |
| <I/σ(I)> | 9.9 | 3.4 |
| Completeness [%] | 94.6 | 83.5 |
| Redundancy | 7.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7 | 293 | Jeffamine M600, 1,2,3-heptanetriol, monoolein, ammonium sulfate, HEPES |
