4TSM
MBP-fusion protein of PilA1 from C. difficile R20291 residues 26-166
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 99.1 |
| Detector technology | PIXEL |
| Collection date | 2014-06-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979470 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.951, 79.366, 164.746 |
| Unit cell angles | 90.00, 90.23, 90.00 |
Refinement procedure
| Resolution | 38.580 - 1.899 |
| Rwork | 0.197 |
| R-free | 0.23260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ogm |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.580 | 1.966 |
| High resolution limit [Å] | 1.899 | 1.899 |
| Rmerge | 0.178 | 1.844 |
| Number of reflections | 139534 | |
| <I/σ(I)> | 1.89 | 1.14 |
| Completeness [%] | 94.9 | 92.48 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 20% PEG2000 MME, 0.2 M trimethylamine N-oxide, 0.1 M Tris-HCl, pH 8.5 |
