4TS2

Crystal structure of the Spinach RNA aptamer in complex with DFHBI, magnesium ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	39.877, 49.399, 188.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.884
R-factor	0.2154
Rwork	0.213
R-free	0.25660
RMSD bond length	0.007
RMSD bond angle	1.732
Data scaling software	SCALA
Refinement software	REFMAC (5.8.0071)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.880
Number of reflections	8415
<I/σ(I)>	23.9
Completeness [%]	99.6
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 3350, 0.5M NaCl, 0.1M succinate-K pH 7.0, 10% sucrose