4TQF

Crystal Structure of the C-terminal domain of IFRS bound with 2-(5-bromothienyl)-L-Ala and ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9202
Spacegroup name	P 64
Unit cell lengths	105.110, 105.110, 70.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.204 - 2.714
R-factor	0.1726
Rwork	0.168
R-free	0.21120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zim
RMSD bond length	0.004
RMSD bond angle	0.926
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.204
High resolution limit [Å]	2.710
Number of reflections	12071
<I/σ(I)>	39.6
Completeness [%]	99.8
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Hepes, MgCl2, PEG3350