4TQD

Crystal Structure of the C-terminal domain of IFRS bound with 3-iodo-L-Phe and ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9789
Spacegroup name	P 64
Unit cell lengths	104.700, 104.700, 70.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.064 - 2.143
R-factor	0.1589
Rwork	0.155
R-free	0.19250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zim
RMSD bond length	0.014
RMSD bond angle	1.450
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.064
High resolution limit [Å]	2.140
Number of reflections	24274
<I/σ(I)>	34.24
Completeness [%]	99.8
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Hepes, MgCl2, PEG3350