4TNW
C. elegans glutamate-gated chloride channel (GluCl) in complex with Fab and POPC in a lipid-modulated conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 453.750, 192.870, 196.140 |
| Unit cell angles | 90.00, 92.27, 90.00 |
Refinement procedure
| Resolution | 58.700 - 3.200 |
| R-factor | 0.2281 |
| Rwork | 0.227 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rhw |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.739 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.700 | 3.240 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.133 | 0.638 |
| Number of reflections | 256166 | |
| <I/σ(I)> | 7.26 | 1.43 |
| Completeness [%] | 92.2 | 83.5 |
| Redundancy | 1.92 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 35-36% pentaerythritol propoxylate (5/4 PO/OH), 50 mM sodium citrate pH 5.5, 100 mM potassium chloride, Crystals were obtained after incubation of protein with lipid and detergent for 4 weeks at 4 degC |
