4TNV
C. elegans glutamate-gated chloride channel (GluCl) in complex with Fab in a non-conducting conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 455.810, 195.680, 196.180 |
Unit cell angles | 90.00, 93.15, 90.00 |
Refinement procedure
Resolution | 58.438 - 3.600 |
R-factor | 0.2622 |
Rwork | 0.261 |
R-free | 0.28290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rhw |
RMSD bond length | 0.004 |
RMSD bond angle | 0.783 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.440 | 3.640 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.155 | 0.563 |
Number of reflections | 175651 | |
<I/σ(I)> | 5.82 | 1.37 |
Completeness [%] | 88.4 | 78.9 |
Redundancy | 1.85 | 1.54 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 35-36% pentaerythritol propoxylate (5/4 PO/OH), 50 mM sodium citrate pH 5.5, 100 mM potassium chloride |