4TNT
Structure of the human mineralocorticoid receptor in complex with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 74.090, 115.136, 81.393 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.231 - 2.394 |
R-factor | 0.2136 |
Rwork | 0.209 |
R-free | 0.25840 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.243 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.084 | 0.074 | 0.438 |
Total number of observations | 80946 | ||
Number of reflections | 14459 | ||
<I/σ(I)> | 17.5 | ||
Completeness [%] | 90.4 | 99.1 | 60.1 |
Redundancy | 5.6 | 6.6 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M sodium malonate, 12 % PEG 3350 |