4TNP
Structural basis of cellular dNTP regulation, SAMHD1-GTP-dCTP-cCTP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.507, 140.753, 97.490 |
| Unit cell angles | 90.00, 114.79, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.22160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bzb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.474 |
| Data reduction software | HKL-2000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.125 | 0.073 | |
| Total number of observations | 419174 | ||
| Number of reflections | 131807 | ||
| <I/σ(I)> | 8.1 | ||
| Completeness [%] | 98.5 | 98.1 | 98.7 |
| Redundancy | 3.2 | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.4 | 298 | SPG buffer, PEG 1500 |
