4TN7

Crystal structure of mouse KDM2A-H3K36ME-NO complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	55.063, 157.928, 48.706
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.166 - 2.200
R-factor	0.232
Rwork	0.229
R-free	0.27940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yu1
RMSD bond length	0.002
RMSD bond angle	0.532
Refinement software	PHENIX ((phenix.refine: dev_1760))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge		0.482
Number of reflections	40902
<I/σ(I)>	9.1	1.8
Completeness [%]	97.9	97.5
Redundancy	2.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM Li-citrate 18-30% PEG 3350