4TMZ

Translation initiation factor eIF5B (517-858) from C. thermophilum, bound to GTPgammaS and potassium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-12
Detector	PSI PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 41 21 2
Unit cell lengths	116.130, 116.130, 120.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.679 - 2.280
R-factor	0.2016
Rwork	0.200
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ncn
RMSD bond length	0.004
RMSD bond angle	1.022
Data reduction software	XDS
Data scaling software	XSCALE (2.5.2)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1690))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.420
High resolution limit [Å]	2.280	10.200	2.280
Rmerge	0.057	0.030	0.540
Rmeas	0.061	0.033	0.646
Total number of observations	307464
Number of reflections	38004	500	2744
<I/σ(I)>	21.43	55.71	3.84
Completeness [%]	99.9	98.2	99.7
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 8000, glycerol, KCl