4TMV

Translation initiation factor eIF5B (517-858) from C. thermophilum, bound to GTPgammaS and Sodium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-18
Detector	PSI PILATUS 6M
Wavelength(s)	0.920
Spacegroup name	P 1 21 1
Unit cell lengths	55.640, 115.850, 66.090
Unit cell angles	90.00, 101.35, 90.00

Refinement procedure

Resolution	43.185 - 1.530
R-factor	0.1646
Rwork	0.163
R-free	0.18790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ncn
RMSD bond length	0.020
RMSD bond angle	1.873
Data reduction software	XDS
Data scaling software	XSCALE (2.5.2)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1690))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		1.630
High resolution limit [Å]	1.530	50.000	1.530
Rmerge			0.496
Rmeas	0.042		0.569
Total number of observations	510862
Number of reflections	121606		20942
<I/σ(I)>	18.76		2.79
Completeness [%]	98.5		98.4
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	HEPES, PEG 4000, NaOAc