4TMT
Translation initiation factor eIF5B (517-858) mutant D533A from C. thermophilum, bound to GTPgammaS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.410, 115.910, 66.090 |
| Unit cell angles | 90.00, 101.37, 90.00 |
Refinement procedure
| Resolution | 46.370 - 1.580 |
| R-factor | 0.1699 |
| Rwork | 0.169 |
| R-free | 0.19350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ncn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.463 |
| Data reduction software | XDS |
| Data scaling software | XSCALE (2.5.2) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1690)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.370 | 1.680 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.515 | |
| Number of reflections | 110416 | |
| <I/σ(I)> | 17.7 | |
| Completeness [%] | 98.8 | 98 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | HEPES, PEG 4000, NaOAc |
